BIO212 AA – Biotechnology I – Nucleic Acid Biochemistry
The first of a 3-part ‘Technical Core’ (BIO212 AA,AB, AC) that trains individuals for a career in the laboratory. BIO212 AA is a hands-on course with up to 15 hours of lab time per week. The course focuses on fundamental techniques in molecular biology including the preparation of chemi-competent E. coli, transformation, plasmid preps, the use of restriction endonucleases, gel electrophoresis and PCR. The course culminates with a gene cloning project. Documentation and experimental design are also components of the course.
Suggested pre-requisite courses: BIO181 and CHM151
Suggested co-requisite course: BIO220.
BIO212 AB – Biotechnology II – Introduction to Protein Biochemistry
The second installment of the biotechnology program core classes. In this lab/lecture course students will dive into the world of proteins. Here they will get hands on experience expressing proteins using inducible plasmids building on their knowledge from BIO212AA. They will then learn and practice several methods of protein isolation, purification, visualization, and quantitation. We will focus on several fundamental protein molecular techniques such as SDS-PAGE, western blotting, salt based precipitation, chromatography, the Bradford and BCA assays. BIO212AB is designed to help students think on their feet (and on their own), troubleshoot, multitask, work in a group environment and be self sufficient in a laboratory setting.
BIO212 AC – Biotechnology III – Mammalian Cell Culture
The final and perhaps most intense course in the biotechnology core. Here students enter the realm of eukaryotic cell culture where they learn how to initiate, maintain, and preserve mammalian cells from adherent cell cultures. We initiate primary murine cultures and attempt to establish fibroblast and myoblast cell lines. This course teaches karyotyping, proper cell culture asepsis, eukaryotic transformation via electroporation, epifluorescence microscopy, RNA isolation, and gene expression analysis via qRT-PCR. Students maintain a course long side project which gives them an introduction into bioinformatics, DNA sequencing, and phylogenetics. Students are also required to read two peer reviewed journal articles and present the data to the class in PowerPoint form.