The Enterotube II is a commercial product available from Becton-Dickinson and Company for identification of enteric (Gram-negative intestinal) bacilli. Its twelve small compartments substitute for twelve test tubes containing appropriate media for these tests: glucose, lysine, ornithine, H₂S/indole, adonitol, lactose, arabinose, sorbitol, Voges-Proskauer, Dulcitol/PA, urea and citrate.

The wire inserted in the Enterotube is used to obtain growth from an isolated colony of the test organism. The wire is pushed down through the twelve compartments, inoculating each test as it proceeds. Results are recorded after 18 to 24 hours of incubation. Reagents are added as needed (e.g. Kovac's reagent is added to the indole test.)

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1. Enterotube ready for inoculation	2. Enterotube results