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EXAMINATION TWO PRACTICE QUESTIONS

  1. True or false: Either F+ or Hfr cells are required to act as the donor if transformation is going to occur.

  2. Because the progeny each receive one old DNA strand and one newly synthesized DNA strand, DNA replication is said to be

  3. The base which forms hydrogen bonds with thymine in DNA is

  4. Coliforms are Gram-positive rods.

  5. Salmonella infections can be acquired through handling pet turtles

  6. Mannitol salt agar is an example of media which is

  7. "Clap" is a street name used for the disease


  8. When a bacteria acquires a new gene that has come floating in solution and migrated into the cell, this process is called

  9. Infections with Salmonella are usually


  10. If a mutation occurs in a segment of DNA, it is most likely to affect

  11. The enzyme used in genetic engineering to open a plasmid and produce "sticky ends" is called a

  12. Restriction endonucleases would be expected to cut

  13. Agrobacterium tumefaciens causes crown gall tumors. It has been used as a genetic engineering tool to move genes into

  14. The following steps must be performed to make a bacterium produce human interferon:

    1-Translation
    2-Restriction endonuclease
    3-Procaryotic transcription
    4 - DNA ligase
    5-Transformation
    6-Eucaryotic transcription
    7 - Reverse transcriptase

    Put the steps in the correct sequence.


  15. Describe how the Polymerase Chain Reaction (PCR) technique could be used to conduct a test for hepatitis B. Identify each specific step used in its proper sequence.

  16. Griffith's experiment with Streptococcus pneumoniae demonstrated that the process of ___________can occur in certain bacteria.

  17. A baby born to a mother with ________ really needs silver nitrate drops or erythromycin put into their eyes soon after birth to prevent blindness.

  18. If gene A mutates at a rate of 10 -2 per cell division, gene B mutates at a rate of 10 -7 per cell division, what is that chance that both genes will mutate in the same cell?
  19. When a nitrogen base is added to the DNA, this causes a ____________ mutation.
  20. ___________, which has been associated with Mexican-style soft cheeses, can cause premature delivery in pregnant women.
  21. Hemophilus influenzae type b is of greatest concern in ________________.
      a. the elderly.
      b. young children.
      c. tuberculosis patients.
      d. patients with urinary tract infections.
      e. syphilis patients.

  22. The first recombinant DNA experiment was performed by Herbert Boyer and Stanley Cohen. In their experiment, they moved a gene from a _______ to a ________ cell.
      a. toad, bacterial
      b. bacterium, toad
      c. virus, bacterial
      d. bacterium, virus
      e. virus, yeast


  23. The symptoms of food poisoning show up very quickly (usually within one to six hours after ingestion) when a person is infected with __________ because this bacteria produces a toxin. Other bacteria responsible for food poisoning usually require at least twelve hours for symptoms to appear because the bacteria must first multiply in the person.
      a. Shigella
      b. Salmonella
      c. Staphylococcus
      d. Neisseria
      e. Proteus


  24. Chlamydia trachomatis is a ________________.
      a. virus
      b. bacteria
      c. fungi
      d. protozoa
      e. metazoa


  25. A "bacteriophage" is a ________________.
      a. bacteria that is infected by a virus
      b. virus that is infected by a bacteria
      c. virus that can infect a bacteria
      d. a bacteria that can infect a virus
      e. none of the above


  26. Sickle cell anemia is due to a _______ mutation in the gene that codes for the beta chain in hemoglobin.
      a. missense
      b. frameshift
      c. nonsense


    ANSWERS TO EXAMINATION TWO PRACTICE QUESTIONS

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